Microplate Readers and Reader Accessories

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.

The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.

In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.

Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.

Features may include:

Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO₂/O₂controls
Modular design for adding functionality

Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.

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361

Revvity Health Sciences Inc HTRF dsRNA (IVT) Detection Kit, 100,000 Assay Points

HTRF dsRNA (IVT) Detection Kit, 100,000 Assay Points

Pricing & Availability

362

Revvity Health Sciences Inc HTRF Human RIG-1 Binding Kit Standard

HTRF Human RIG-1 Binding Kit Standard

Pricing & Availability

363

Revvity Health Sciences Inc HTRF CD32a (131R) Cellular Binding Kit, 1,000 Assay Points

HTRF CD32a (131R) Cellular Binding Kit, 1,000 Assay Points

Pricing & Availability

364

Molecular Devices Fluorescence Polarization Filter, 700 nm, 60 nm, Horizontal

This fluorescence polarization filter is configured for near-infrared polarized fluorescence measurements and is optimized for a 700 nm center wavelength with a 60 nm bandwidth. It installs in a reader filter slot to enable polarization-based assays and filter-based detection in compatible multi-mode microplate readers.

Provides 700 nm center wavelength with 60 nm bandwidth.
Polarized horizontal orientation for fluorescence polarization assays.
Installs in a standard filter slot or filter holder for multi-mode readers.
Optimizes sensitivity for near-IR fluorophores and polarization-based detection.
Store in a dust-free environment and avoid touching optical surfaces.